产品名称
PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20), liquid, clone PY20, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
PY20, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1:500-1:1000,
Frozen Sections
Immunocytochemistry
Immunoprecipitation )
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr)
Quality Level
Gene Information
human ... PID1(55022)
Analysis Note
Positive Control
Any cell lysate or tissue extract
Any cell lysate or tissue extract
Application
ELISA (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.
Immunogen
KLH conjugated to phosphotyrosine, glycine, and alanine
Other Notes
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM sodium phosphate, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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