生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
PY20, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性（根据同源性预测）
all
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1:500-1:1000,
Frozen Sections
Immunocytochemistry
Immunoprecipitation )
同位素/亚型
IgG2b
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pTyr)
基因信息
human ... PID1(55022)
一般描述
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.
免疫原
KLH conjugated to phosphotyrosine, glycine, and alanine
应用
ELISA (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
包装
Please refer to vial label for lot-specific concentration.
外形
In 10 mM sodium phosphate, pH 7.4.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Any cell lysate or tissue extract
Any cell lysate or tissue extract
其他说明
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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