527460
PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb
liquid, Calbiochem®
别名:
Anti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Species reactivity:
human, mouse
Clone:
polyclonal
Application:
—
Citations:
3
biological source
rabbit
Quality Segment
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
phosphorylation (pThr451)
Gene Information
human ... EIF2AK2(5610)
General description
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
Application
Immunoblotting (1:1000)
Immunohistochemistry (see comments)
Immunohistochemistry (see comments)
Physical form
In Dulbecco′s PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Analysis Note
Positive Control
IFNγ-stimulated HeLa cells.
IFNγ-stimulated HeLa cells.
Other Notes
Cuddihy, A.R., et al.1999. Oncogene8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.
When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable