Prion Protein, His•Tag^®, Human, Recombinant, E. coli

Recombinant, human prion protein (amino acids 23-231) fused to a His•Tag sequence and expressed in E. coli. Prion diseases or transmissible spongiform encephalopathies are neurodegenerative diseases that affect both humans and animals. All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non-ionic detergent and partially resistant to proteases (PrPsc). Useful as an antigen standard in immunochemical detection of Creutzfeldt-Jakob Disease (CJD).

In 10 mM sodium acetate, pH 4.0.

Following initial thaw, aliquot and freeze (-20°C).

Calzolai, L., and Zahn, R. 2003. J. Biol. Chem.278, 35592.
Prusiner, S.B. 1998. Proc. Natl. Acad. Sci. USA95, 13363.
Swietnicki, W., et al. 1997. J. Biol. Chem.272, 27517.
Pan, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10962.
Basler, K., et al. 1986. Cell46, 417.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)