5.30650
Luciferase Substrate, CycLuc1
别名:
Luciferase Substrate, CycLuc1, 2-(6,7-dihydro-5h-thiazolo[4,5-f]indol-2-yl)-4,5-dihydrothiazole-4-carboxylicacid
storage temp.
2-8°C
assay
≥94% (HPLC)
form
powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
yellow
solubility
DMSO: 100 mg/mL
Quality Level
General description
A synthetic cyclic alkylamino luciferin that exhibitsproperties that are superior to those of D-luciferin. Binds to luciferase with higheraffinity and emits about 5.7-fold more light than aminoluciferin and3.2-fold more light than D-luciferin. Its superior light output is maintainedover a wide range (1-100 µM). Shown to readily cross the blood-brain barrier inmice and provides much improved noninvasive bioluminescence imaging (BLI) ofthe brain at a lower dose when compared to D-luciferin. Yields about 10-foldhigher bioluminescent signal than D-luciferin when Luc2 luciferase expressing4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice.A rapid (4-5 min), long-lasting, and steady signal peak is observed followingan i.v injection of CycLuc1 in mice.
Application
Luciferase Substrate, CycLuc1 has been used for transfecting HEK293 cells to study the inhibition of intracellular luciferase crystallization by D-luciferin and its synthetic analogues.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Luciferase
Luciferase
Packaging
Packaged under inert gas
Other Notes
Please note that the molecular weight for this compound is batch-specific due to variable water content.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Haruki Hasegawa
Experimental cell research, 440(1), 114131-114131 (2024-06-15)
Firefly luciferase (Fluc) from Photinus pyralis is one of the most widely used reporter proteins in biomedical research. Despite its widespread use, Fluc's protein phase transition behaviors and phase separation characteristics have not received much attention. Current research uncovers Fluc's
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