MMP-7, Proenzyme, Human, Recombinant, E. coli

Recombinant, human pro-MMP-7 expressed in E. coli. MMP-7 is believed to be a key factor in the regulation of metastasis. Overexpression of MMP-7 is observed in colon cancer. Activatable by organomercurials (APMA; Cat. No. 164610).

Please refer to vial label for lot-specific concentration.

In 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, 0.02% NaN₃, 0.01% Brij^® 35 Detergent, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C). Freeze-thaw cycles may accelerate the activation process.

Girolamo, N. D., et al. 2001. Invest. Ophtal. Visual. Sci.42, 1963.
Kihira, Y., et al. 1996. Urol. Oncol. 2, 20.
Woessner, J.F. 1995. Methods Enzymol. 248, 485.
Crabbe, T., et al. 1992. Biochemistry 31, 8500.

One unit is defined as the amount of enzyme that will digest 1.0 µg AZOCOLL Substrate (Cat. No. 194933), in the presence of 500 μM p-aminophenylmercuric acetate (APMA; Cat. No. 164610) per minute at 37°C, pH 7.5.

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)