Merck
CN
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文件

539134

Millipore

蛋白酶抑制剂混合物套装III,无EDTA

Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.

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别名:
EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III
NACRES:
NA.77

形式

liquid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

运输

wet ice

储存温度

−20°C

一般描述

注意:以1 x 1 ml或一组5 x 1 ml形式提供。
该蛋白酶抑制剂混合物是由六种蛋白酶抑制剂组成的特殊混合物,具有广泛的特异性,可抑制天冬氨酸、半胱氨酸和丝氨酸蛋白酶以及氨基肽酶。建议将该混合物与哺乳动物细胞和组织提取物一起使用,但也适用于细菌提取物。它以单个试剂瓶或一组5个试剂瓶的形式提供。每个小瓶包含1 ml蛋白酶抑制剂混合物,该混合物溶于含有少量水的DMSO中。

特异性

广泛抑制丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶以及氨基肽酶。

应用

Protease Inhibitor Cocktail Set III, EDTA-Free has been used:
  • as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis
  • as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue
  • in lysis buffer to extract proteins for immunoblotting

生化/生理作用

Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.

特点和优势

  • EDTA-Free: recommended for IMAC applications.
  • Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
  • Fast: no waiting time for tablets to dissolve.
  • Economical: ready-to-use format reduces wastage.
  • Effective: one ml is sufficient for approximately 20g of tissue.
  • Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.

警告

毒性:刺激性(B)

重悬

初次融化后,等分并冷冻(-20°C)。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

188.6 °F - (refers to pure substance)

闪点(°C)

87 °C - (refers to pure substance)


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Montalbetti N, et al.
American Journal of Physiology: Renal Physiology, 323(4), F479-F491 (2022)
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Journal of Cellular Physiology, 234, 13068-13082 (2019)
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Nature Protocols, 9, 64-75 (2014)

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