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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
guinea pig, Xenopus, sheep, bovine, rat, canine, hamster, porcine, mouse, monkey, human
Application:
Citations:
3
产品名称
Anti-Protein Disulfide Isomerase Rabbit pAb, liquid, Calbiochem®
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≥0.9% sodium azide as preservative
species reactivity
guinea pig, Xenopus, sheep, bovine, rat, canine, hamster, porcine, mouse, monkey, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:1000, colorimetric)
Immunocytochemistry
Immunoprecipitation
Paraffin Sections )
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PDIA2(64714)
Analysis Note
Positive Control
HeLa cells, rat testis, mouse spleen
HeLa cells, rat testis, mouse spleen
Application
Immunoblotting (1:1000, colorimetric)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Protein A purified rabbit polyclonal antibody. Recognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
Immunogen
Bovine
bovine liver Protein Disulfide Isomerase
Other Notes
Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther.282, 1064.
Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
This antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.
Physical form
In PBS, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Lihe Zhang et al.
Metabolism: clinical and experimental, 57(8), 1115-1124 (2008-07-22)
High fructose consumption is associated with the development of fatty liver and dyslipidemia with poorly understood mechanisms. We used a matrix-assisted laser desorption/ionization-based proteomics approach to define the molecular events that link high fructose consumption to fatty liver in hamsters.
Anthony O Gramolini et al.
Molecular & cellular proteomics : MCP, 7(3), 519-533 (2007-12-07)
Defective mobilization of Ca2+ by cardiomyocytes can lead to cardiac insufficiency, but the causative mechanisms leading to congestive heart failure (HF) remain unclear. In the present study we performed exhaustive global proteomics surveys of cardiac ventricle isolated from a mouse
Alvaro Souto Padron de Figueiredo et al.
The Journal of biological chemistry, 290(21), 13427-13439 (2015-04-01)
Inflammation and oxidative stress through the production of reactive oxygen species (ROS) are consistently associated with metabolic syndrome/type 2 diabetes. Although the role of Nox2, a major ROS-generating enzyme, is well described in host defense and inflammation, little is known
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