Protein G, Alkaline Phosphatase Conjugate

Protein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.

Protein G, Alkaline Phosphatase Conjugate is obtained by conjugation of protein G, a cell-surface protein of Streptococcus species to alkaline phosphatase. This conjugate is used as a secondary agent in immunoassay methods to detect polyclonal goat and rabbit antibodies and monoclonal mouse IgG1, IgG2a, and IgG2b.

Protein G, Alkaline Phosphatase Conjugate has been used in:

• direct enzyme-linked immunosorbent assay (ELISA) (1:1000)(1:250)
• indirect ELISA (1:1000)
• immunoblot (1:5000)

Please refer to vial label for lot-specific concentration.

Toxicity: Standard Handling (A)

In 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.

Single band at 33.8 kDa by SDS-PAGE

p-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl₂ at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

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