Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P_i into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

In 50 mM Tris-HCl, pH 7.6.

Single band by SDS-PAGE

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.

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