产品名称
Anti-Ras (31-43) Rabbit pAb, liquid, Calbiochem®
Quality Level
biological source
rabbit
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, bovine, human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1 µg/mL))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAB2A(5862)
Analysis Note
Positive Control
Rat brain tissue
Rat brain tissue
Application
Immunoblotting (1 µg/mL)
Disclaimer
Toxicity: Standard Handling (A)
General description
Immunoaffinity rabbit polyclonal antibody. Recognizes all forms of wild type Ras and the mutant G12V Ras protein.
Recognizes all forms of wild type Ras, including the mutant G12V Ras protein.
This Anti-Ras (31-43) Rabbit pAb is validated for use in Immunoblotting for the detection of Ras (31-43).
Immunogen
a synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH
Other Notes
By immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
Hardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418,Academic Press, San Diego, CA.
Sawada, M., et al. 1989. Nucleic Acid Res.17, 8867.
Sawada, M., et al. 1989. Nucleic Acid Res.17, 8867.
Packaging
Please refer to vial label for lot-specific concentration.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Björn Handke et al.
PloS one, 9(9), e107333-e107333 (2014-09-10)
The wing imaginal disc of Drosophila melanogaster is a prominent experimental system for research on control of cell growth, proliferation and death, as well as on pattern formation and morphogenesis during organogenesis. The precise genetic methodology applicable in this system
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