生物来源
goat
表单
lyophilized solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG
溶解性
soluble (Reconstitute in 10 ml of H2O. If the solution is not clear after 1-2 h standing at room temperature, centrifuge and transfer the supernatant to clean tubes.)
运输
wet ice
储存温度
2-8°C
一般描述
Normal carrier sera, produced from pooled lots of sera from healthy, non-immunized goats. Suitable for use in radioimmunoassay.
应用
Normal Goat Serum, Lyophilized Solid has been used as a blocking agent in:
- immunohistochemistry
- immunocytochemistry
- immunofluorescence
生化/生理作用
Normal goat serum can be used as an alternative to commonly used sera such as fetal bovine serum (FBS) and newborn calf serum (NBCS) for the primary culture of cells from various animals. In addition, goat serum also has the potential to use for routine culture of fish cells.
外形
Lipid extracted, dialyzed against 250 mM NaCl, 10 mM sodium phosphate, 0.05% sodium azide, pH 7.6, and lyophilized.
制备说明
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Undiluted stock solutions are stable for several weeks at 4°C or for up to 1 year at -20°C. Avoid freeze/thaw cycles. Do not stored diluted stock solutions for more than 1 day at 4°C.
Reconstitute in 10 ml of H₂O. If the solution is not clear after 1-2 h standing at room temperature, centrifuge and transfer the supernatant to clean tubes.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
警示用语：
Warning
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
储存分类代码
13 - Non Combustible Solids
WGK
WGK 1
法规信息
新产品
P K Nanda et al.
In vitro cellular & developmental biology. Animal, 45(3-4), 148-151 (2009-01-02)
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International journal of molecular medicine, 41(3), 1455-1462 (2018-01-13)
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Tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) is known to induce cell apoptosis in many types of cancer cells. However, some malignant cells still exhibit anti‑apoptosis features induced by TRAIL; thus the underlying mechanisms that regulate sensitivity and resistance of tumor
Effects of Gallic Acid on Endometrial Cancer Cells in Two and Three Dimensional Cell Culture Models.
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Cell culture studies are an indispensable tools used to understand basic physiological, biophysical and biomolecular mechanisms. Although traditional two-dimensional (2D) cell culture models are more preferred in experimental studies, three-dimensional (3D) cell culture models, attract more attention due to several
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