Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb

Recognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD.

Sheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the Cu/Zn superoxide dismutase protein.

Superoxide dismutase Cu/Zn enzyme (SOD (Cu/Zn)) is a homodimeric metalloenzyme of aerobic organisms, comprised of subunits of ~16 kDa. Found in a variety of cell types, including erythrocytes, liver, and brain, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O₂-) to molecular oxygen (O₂) and hydrogen peroxide (H₂O₂).

This Anti-Superoxide Dismutase Sheep pAb is validated for use in WB, Immunoelectrophoresis, Frozen sections, Paraffin sections, Free-floating sections for the detection of Superoxide Dismutase.

Human

highly purified, human erythrocyte SOD Cu/Zn.

Immunoblotting (1:1000)

Immunoelectrophoresis (see comments)

Frozen sections (see comments)

Paraffin sections (see comments)

Free-floating sections (see comments)

Please refer to vial label for lot-specific concentration.

In glycine-buffered saline, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
Human red blood cell lysate

Pasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15763.

This antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)