biological source
human plasma
description
Merck USA index - 14, 9383
form
lyophilized
specific activity
≥1000 NIH units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: 1 mg/mL
aqueous buffer: soluble
storage temp.
−20°C
Quality Level
Analysis Note
通过SDS-PAGE从均质凝血酶原完成激活
Application
人血浆凝血酶已用于：
- 作为内皮生长培养基（EGM）成分用于卡波氏肉瘤相关疱疹病毒人内皮细胞系（KSHV-HuARLT）小鼠细胞的移植和再分离
- 生产纤维蛋白凝胶
- 作为EGM培养基成分用于KSHV-HuARLT细胞和matrigel植入物的病毒拷贝数分析
Biochem/physiol Actions
凝血酶裂解纤维蛋白原，并将其转化为纤维蛋白。然后它激活因子V、VIII、XI和XIII。凝血酶可刺激血小板活化，并稳定纤维蛋白聚合物。它在凝血级联系统的最后阶段起着关键作用。
Disclaimer
仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
毒性：有害（C）
General description
凝血酶是一种钠激活的二型酶，包含两个阴离子结合的外源性位点，即ABE-I和ABE-II。这种丝氨酸蛋白酶由酶原凝血酶原（因子II）在肝脏中合成。
Other Notes
一个单位是通过与使用生物制品局标准凝血酶生成的标准曲线的比较来测定的。
Physical form
从200 mM NaCl、50 mM枸橼酸盐缓冲液、0.1% PEG-8000（pH 6.5）中冷冻干燥。含有BSA作为稳定剂。
Preparation Note
复溶后，等分并冷冻保存（-70°C）。贮备溶液在-70°C下可稳定保存至多2个月。
由经认证测试对HBsAg以及HIV和HCV抗体呈阴性的血浆制备而成。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
高风险级别生物产品-Merck
此项目有
Kitchens CS, et al
Consultative Hemostasis and Thrombosis (2013)
Segall JA and Liem TK
Congenital and Acquired Hypercoagulable Syndromes, 339-346 (2007)
Isis S R Carter et al.
Thrombosis, 2010, 416167-416167 (2010-01-01)
Although prothrombin is one of the most widely studied enzymes in biology, the role of the thrombin A-chain has been neglected in comparison to the other domains. This paper summarizes the current data on the prothrombin catalytic domain A-chain region
Diana L Diesen et al.
Vascular, 16 Suppl 1, S29-S36 (2008-03-01)
Thrombin is a common hemostatic drug used in surgical practice for over 100 years because of its simplicity and efficacy. Thrombin converts fibrinogen to fibrin, activates platelets, and induces vascular contraction. It is available in multiple forms, including human thrombin
Madhavi A Jadhav et al.
Biochemistry, 49(13), 2918-2924 (2010-03-12)
The formation of a blood clot involves the interplay of thrombin, fibrinogen, and Factor XIII. Thrombin cleaves fibrinopeptides A and B from the N-termini of the fibrinogen Aalpha and Bbeta chains. Fibrin monomers are generated that then polymerize into a
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