生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
Ab33, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, porcine, mouse, rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections
Immunoblotting (0.2-1 µg/mL, chemiluminescence)
Immunoprecipitation
Paraffin Sections (not recommended))
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TEK(7010)
一般描述
Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
Recognizes the ~140-45 kDa Tie2 protein.
This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.
免疫原
Human
a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag sequence
应用
Frozen Sections (see comments)
Immunoblotting (0.2-1 µg/ml, chemiluminescence)
Immunoprecipitation (see comments)
Paraffin Sections (not recommended)
Immunoblotting (0.2-1 µg/ml, chemiluminescence)
Immunoprecipitation (see comments)
Paraffin Sections (not recommended)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
外形
In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-20°C).
其他说明
For frozen sections, tissues were treated with Triton™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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