616382
Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride
≥95% (elemental analysis), trypsin like proteases inhibitor, solid
别名:
Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride, TLCK
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关于此项目
经验公式（希尔记法）:
C14H21ClN2O3S · xHCl
化学文摘社编号:
分子量:
332.85 (free base basis)
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
产品名称
Nα-Tosyl-Lys Chloromethyl Ketone, Hydrochloride, Inhibits trypsin-like serine proteinases.
SMILES string
[S](=O)(=O)(N[C@@H](CCCC[N+H3])C(=O)CCl)c1ccc(cc1)C.[Cl-]
InChI
1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1
InChI key
YFCUZWYIPBUQBD-ZOWNYOTGSA-N
description
RTECS - XT5160000
assay
≥95% (elemental analysis)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic)
color
off-white
solubility
aqueous solution, pH <6.0: 1 mg/mL
shipped in
ambient
Quality Level
General description
Inhibits trypsin-like serine proteases. Inactivates trypsin irreversibly without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC50 = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemic cells and normal thymocytes. Suggesting working concentration is 10-100 µM.
Inhibits trypsin-like serine proteinases. Irreversibly inactivates trypsin without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to the target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC50 = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemia cells and normal thymocytes.
Biochem/physiol Actions
Cell permeable: no
EC50 = 80 µM blocking nitric oxide synthase induced by interferon-γ and lipopolysaccharides in macrophages
Primary Target
trypsin like proteases
trypsin like proteases
Product does not compete with ATP.
Reversible: no
Preparation Note
Unstable in solution; reconstitute just prior to use.
Other Notes
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Kim, H., et al. 1995. J. Immunol.154, 4741.
Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
Biro, A., et al. 1992. Eur. J. Immunol.22, 2547.
Bruno, S., et al. 1992. Leukemia6, 1113.
Lee, S.F. 1992. Infect. Immun. 60, 4032.
Schmidt, H.H., et al. 1992. Mol. Pharmacol.41, 615.
Kim, H., et al. 1995. J. Immunol.154, 4741.
Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
Biro, A., et al. 1992. Eur. J. Immunol.22, 2547.
Bruno, S., et al. 1992. Leukemia6, 1113.
Lee, S.F. 1992. Infect. Immun. 60, 4032.
Schmidt, H.H., et al. 1992. Mol. Pharmacol.41, 615.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1B
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