产品名称
Anti-TNF-α Rabbit pAb, liquid, Calbiochem®
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse (weakly)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA
Frozen Sections
Immunoblotting
Neutralization Studies
Paraffin Sections
Radioimmunoassay )
impurities
≤1 EU/mg Endotoxins
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TNF(7124)
Immunogen
recombinant, human TNF-α
Other Notes
1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
Reacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
Analysis Note
Positive Control
L929 cells
L929 cells
Application
ELISA (see comments)
Frozen Sections (see comments)
Immunoblotting (see comments)
Neutralization Studies (see comments)
Paraffin Sections (see comments)
Radioimmunoassay (see comments)
Frozen Sections (see comments)
Immunoblotting (see comments)
Neutralization Studies (see comments)
Paraffin Sections (see comments)
Radioimmunoassay (see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
Recognizes recombinant and monocyte-derived TNF-α. Exhibits some cross-reactivity with lymphotoxin (TNF-β) and mouse TNF-α in L929 cells.
This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.
Packaging
Please refer to vial label for lot-specific concentration.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Josefa P Alameda et al.
Aging, 11(1), 127-159 (2019-01-12)
CYLD is a deubiquitinating enzyme known for its role as a tumor suppressor whose mutation leads to skin appendages tumors and other cancers. In this manuscript we report that the tumor suppressor CYLD, similarly to other renowned tumor suppressor genes
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