Application
Ubiquitin, GST-Fusion, Recombinant, E. coli has been used for in vivo and in vitro ubiquitination assays, to study the role of FBXL2-Grp94-EGFR axis in non-small cell lung cancer (NSCLC) development and the role of F-box protein 3 (FBXO3) in promotion of breast cancer metastasis.
Biochem/physiol Actions
The ubiquitin (Ub) system plays a crucial role in protein homeostasis by maintaining protein balance in pathways related to DNA repair, cell cycle progression, apoptosis (programmed cell death), receptor-mediated endocytosis, and signal transduction. Imbalances or dysfunctions within this system can lead to pathological conditions. Apart from this, disruptions in ubiquitin pathways can also contribute to the development of human diseases, including neurodegenerative conditions and tumors.
Disclaimer
Toxicity: Standard Handling (A)
General description
Research area: Cancer
Recombinant ubiquitin fused at the N-terminus to GST and expressed in E. coli. Useful for glutathione affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using GST antibodies. Facilitates the visualization of polyUb chains due to larger ladder intervals.
Recombinant ubiquitin fused at the N-terminus to GST and expressed in E. coli. Useful for glutathione affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using GST antibodies. Facilitates the visualization of polyUb chains due to larger ladder intervals.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 50 mM HEPES, pH 8.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Ubiquitin
Kona F.R, et al.
Brenner and Rector's The Kidney E-book, 242-245 (2013)
Metalloregulation of Protein Clearance: New Therapeutic Avenues for Neurodegenerative Diseases
Munoz ET, et al.
Abstr. Soc. Neurosci (2017)
Ubiquitin networks in cancer
Kirkin V and Dikic I
Current Opinion in Genetics & Development, 21(1), 21-28 (2011)
Mengmeng Niu et al.
PLoS biology, 19(2), e3001113-e3001113 (2021-02-25)
Transforming growth factor-β (TGF-β) signaling plays a critical role in promoting epithelial-to-mesenchymal transition (EMT), cell migration, invasion, and tumor metastasis. ΔNp63α, the major isoform of p63 protein expressed in epithelial cells, is a key transcriptional regulator of cell adhesion program
Mengmeng Niu et al.
Nature communications, 12(1), 5919-5919 (2021-10-13)
Abnormal activation of epidermal growth factor receptor (EGFR) drives non-small cell lung cancer (NSCLC) development. EGFR mutations-mediated resistance to tyrosine-kinase inhibitors (TKIs) is a major hurdle for NSCLC treatment. Here, we show that F-box protein FBXL2 targets EGFR and EGFR
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