676604
PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)
lyophilized, clone 5C6, Calbiochem®
别名:
Anti-Vasodilator-stimulated Phosphoprotein, Activated
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
5C6, monoclonal
Species reactivity:
human, mouse
Application:
—
Citations:
1
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
5C6, monoclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA (0.05 µg/mL)
Immunoblotting (1 µg/mL, colorimetric; 0.1 µg/mL, chemiluminescence)
Immunocytochemistry (1-10 µg/mL)
Immunoprecipitation (1-10 µg/10 vanadate-treated A431 cells))
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer157)
Gene Information
human ... VASP(7408)
General description
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
This PhosphoDetect
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
Application
ELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Physical form
Lyophilized from PBS, PEG, sucrose.
Preparation Note
Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Vanadate-treated A431 cells
Vanadate-treated A431 cells
Other Notes
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable