生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
5C6, monoclonal
表单
lyophilized
包含
≤0.1% sodium azide as preservative
种属反应性
human, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
dilution
(ELISA (0.05 µg/mL)
Immunoblotting (1 µg/mL, colorimetric; 0.1 µg/mL, chemiluminescence)
Immunocytochemistry (1-10 µg/mL)
Immunoprecipitation (1-10 µg/10 vanadate-treated A431 cells))
同位素/亚型
IgG1
运输
ambient
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer157)
基因信息
human ... VASP(7408)
一般描述
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
This PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
应用
ELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
外形
Lyophilized from PBS, PEG, sucrose.
制备说明
Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
分析说明
Positive Control
Vanadate-treated A431 cells
Vanadate-treated A431 cells
其他说明
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Min Li et al.
The Journal of biological chemistry, 290(50), 30030-30041 (2015-10-27)
Diabetes is strongly associated with cognitive decline, but the molecular reasons are unknown. We found that fasting and peripheral insulin promote phosphorylation and dephosphorylation, respectively, of specific residues on brain proteins including cytoskeletal regulators such as slit-robo GTPase-activating protein 3
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