684274
β1,2-Xylosidase, Xanthomonas sp.
别名:
β1,2-Xylosidase, Xanthomonas sp., β-D-Xylanxylohydrolase
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Specific activity:
≥10 units/mL, ≥20 units/mg protein
form
liquid
specific activity
≥10 units/mL, ≥20 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
β-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases, none detected
shipped in
wet ice
Quality Level
General description
Native β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.
Note: 1 mU = 1 milliunit.
Physical form
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Preparation Note
Following initial use, refrigerate (4°C).
Other Notes
One unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.
Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol.50, 1401.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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