688225
Caspase-1 Substrate VI, Fluorogenic
Fluorogenic caspase-1 substrate.
别名:
Caspase-1 Substrate VI, Fluorogenic, Z-YVAD-AFC
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关于此项目
经验公式（希尔记法）:
C33H36F3N5O10
化学文摘社编号:
分子量:
719.66
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77
SMILES string
FC(F)(F)c1c2c([o][c](c1)=O)cc(cc2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)Cc3ccc(cc3)O)C(C)C)C)CC(=O)O
InChI
1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1
InChI key
GWKJISJKNKIJNP-DEQDFGQZSA-N
assay
98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated, protect from light
color
white to off-white
solubility
DMSO: 5 mg/mL
fluorescence
λex ~400 nm, λem ~505 nm
shipped in
ambient
storage temp.
−20°C
Quality Level
General description
Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.
Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Fluorogenic caspase-1 substrate
Fluorogenic caspase-1 substrate
Product does not compete with ATP.
Reversible: no
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Ac-Tyr-Val-Ala-Asp-AFC
Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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