form
liquid
manufacturer/tradename
Novagen®
storage condition
do not freeze
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
The T7•Tag Antibody Agarose is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (i.e., the amino terminal 11 aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag monoclonal antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be recycled a minimum of five times without loss of binding activity.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Satoshi Hirano et al.
Autophagy, 12(2), 312-326 (2016-02-24)
MAP1LC3/LC3 (a mammalian ortholog family of yeast Atg8) is a ubiquitin-like protein that is essential for autophagosome formation. LC3 is conjugated to phosphatidylethanolamine on phagophores and ends up distributed both inside and outside the autophagosome membrane. One of the well-known
Sara R Heras et al.
Nature structural & molecular biology, 20(10), 1173-1181 (2013-09-03)
More than half of the human genome is made of transposable elements whose ongoing mobilization is a driving force in genetic diversity; however, little is known about how the host regulates their activity. Here, we show that the Microprocessor (Drosha-DGCR8)
Kazufumi Ohshiro et al.
Genes & cancer, 11(1-2), 43-52 (2020-06-25)
Recently, we observed that the TGF-β pathway is altered in 39% of HCCs. The alterations are correlated with a raised HMGA2 level. Therefore, we compared genetic alterations of HMGA2 and 43 TGF-β pathway core genes in HCC patients from TCGA
Subhashini Sadasivam et al.
Genes & development, 26(5), 474-489 (2012-03-07)
Cell cycle progression is dependent on two major waves of gene expression. Early cell cycle gene expression occurs during G1/S to generate factors required for DNA replication, while late cell cycle gene expression begins during G2 to prepare for mitosis.
Koutaro Ishibashi et al.
Autophagy, 7(12), 1500-1513 (2011-11-16)
A large protein complex consisting of Atg5, Atg12 and Atg16L1 has recently been shown to be essential for the elongation of isolation membranes (also called phagophores) during mammalian autophagy. However, the precise function and regulation of the Atg12–5-16L1 complex has
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