The T7-Tag^® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as PEPscreen^® and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select^® Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.

T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal

Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates

Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)

Working dilution- 1:5,000–1:10,000

Plastic ampoule

Stabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

PEPscreen is a registered trademark of Sigma-Aldrich Co. LLC

T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany

T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany

Multiple Toxicity Values, refer to MSDS