产品名称
Benzonase®核酸酶，纯度> 90%,
biological source
Serratia marcescens
recombinant
expressed in E. coli
assay
>90% (SDS-PAGE)
form
buffered aqueous glycerol solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
25-29 units/μL
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
用于去除蛋白样品中的核酸。
Biochem/physiol Actions
消化天然或热变性的DNA和RNA。
Disclaimer
毒性：标准处理（A）
General description
Benzonase® 核酸酶是一种用基因工程方法从粘质沙雷氏菌获得的核酸内切酶它降解所有形式的 DNA 和 RNA（单链、双链、线性和环状），而没有蛋白水解活性。它能够在较大范围的条件下工作，并且具有极高的比活度。该酶将核酸完全消化为长度为 2 至 5 个碱基（低于杂交极限）的 5′-一磷酸酯化的寡核苷酸，非常适合从重组蛋白中去除核酸，从而符合 FDA 关于核酸污染的指南。Benzonase 迅速水解核酸的能力使该酶成为降低粘度、减少加工时间并提高蛋白质产量的绝佳选择。例如，该酶与 BugBuster 和 PopCulture 蛋白提取试剂兼容，因此可以与这些试剂一起添加，以消除大肠杆菌提取物中的粘度并去除核酸。
该酶包括两个 30kDa 的亚基。它在 pH 值 6-10 之间，温度 0-42°C 起作用，并需要 1-2mM Mg2+ 激活。该酶在离子和非离子去污剂、还原剂、PMSF（1 mM）、EDTA（1 mM）和尿素（相对活性取决于特定条件）的存在下也具有活性。>150 mM 单价阳离子，>100 mM 磷酸盐，>100 mM 硫酸铵或 >100 mM 盐酸胍能抑制其活性。
可提供超纯 Benzonase 核酸酶（>99%，SDS-PAGE）和纯 Benzonase 核酸酶（>90%），标准浓度为 25U/µl，高浓度（HC）为 250U/lµ。两种制剂均不含可检测的蛋白酶，并且比活度 > 1 × 106 蛋白。纯度>99%的产品经过内毒素测试，并含有<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >90%（SDS-PAGE）
该酶包括两个 30kDa 的亚基。它在 pH 值 6-10 之间，温度 0-42°C 起作用，并需要 1-2mM Mg2+ 激活。该酶在离子和非离子去污剂、还原剂、PMSF（1 mM）、EDTA（1 mM）和尿素（相对活性取决于特定条件）的存在下也具有活性。>150 mM 单价阳离子，>100 mM 磷酸盐，>100 mM 硫酸铵或 >100 mM 盐酸胍能抑制其活性。
可提供超纯 Benzonase 核酸酶（>99%，SDS-PAGE）和纯 Benzonase 核酸酶（>90%），标准浓度为 25U/µl，高浓度（HC）为 250U/lµ。两种制剂均不含可检测的蛋白酶，并且比活度 > 1 × 106 蛋白。纯度>99%的产品经过内毒素测试，并含有<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >90%（SDS-PAGE）
Other Notes
一个单位定义为在 30 分钟内导致 ΔA₂₆₀ 为 1.0 的酶量，这对应于 37 µg DNA 的完全消化。
Legal Information
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 2
法规信息
常规特殊物品
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