BugBuster^® Ni-NTA His•Bind^®纯化试剂盒

This article shows the use of BugBuster^® and Benzonase^® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.