一般描述
The FastPlax Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.
包装
Plastic ampoule
制备说明
Multiple storage requirements
其他说明
- 2 × 50 ml- 10X TBST (100 mM Tris-HCl, pH 8.0, 1.5 M NaCl, 1% Tween® 20)
- 6 ml- 10% Gelatin (contains 0.02% azide)
- 10 µl- FastPlax Antibody
- 300 µl- Goat-Anti-Mouse β-Galactosidase Conjugate
- 300 µl- NBT (83 mg/ml in 70% dimethylformamide)
- 300 µl- X-gal (50 mg/ml in dimethylformamide)
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
免责声明
Toxicity: Multiple Toxicity Values, refer to MSDS
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
储存分类代码
3 - Flammable liquids
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