Rapid purification of PCR products for downstream procedures

The SpinPrep<TMSYMBOL></TMSYMBOL> PCR Clean-Up Kit is designedfor the rapid purification of DNA amplifiedin PCR. The 10-minute procedure involvesaddition of a binding buffer followed byadsorption of the DNA to a silica membrane ina spin column format. Following a wash step,the DNA is eluted in low-salt buffer. This kitremoves DNA polymerases, dNTPs, salts, and> 99% of primers so that they do not interferewith downstream applications such as cloning,sequencing, or labeling. PCR products from100 bp to > 12,000 bp can be cleaned up, withrecoveries of amplified DNA in the range of60-90% under standard conditions.
Column binding capacity: up to 6 g
PCR volume: 100 l/rxn
Typical recovery: 6090%
Size range: 100 bp to > 12,000 bp
Time required:< 10 min

•82 mlSpinPrep Bind Buffer

•27 mlSpinPrep Wash Buffer

•10 mlSpinPrep Elute Buffer

•100SpinPrep Filters

•100Receiver Tubes

•100Eluate Receiver Tubes

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Multiple Toxicity Values, refer to MSDS (O)