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Merck
CN

71206

Benzonase® Nuclease HC

Purity > 99%, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

别名:

内切核酸酶 来源于粘质沙雷氏菌

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化学文摘社编号:
9025-65-4
EC 号:
3.1.30.2 (BRENDA, IUBMB)
MDL编号:
MFCD00131010
UNSPSC代码:
12352202
NACRES:
NA.54

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产品名称

Benzonase® 核酸酶HC，纯度> 99%, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

生物来源

Serratia marcescens

质量水平

100

重组

expressed in E. coli

方案

>99% (SDS-PAGE)

表单

buffered aqueous glycerol solution

制造商/商品名称

Novagen®

储存条件

OK to freeze

浓度

≥250 units/μL

杂质

<0.25 EU/kU Total endotoxin

应用

research use

运输

wet ice

储存温度

−20°C

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相关类别

一般描述

Benzonase® 核酸酶是一种用基因工程方法从粘质沙雷氏菌获得的核酸内切酶。它降解所有形式的DNA和RNA（单链、双链、线性和环状），而没有蛋白水解活性。它能够在较大范围的条件下工作，并且具有极高的比活度。该酶将核酸完全消化为长度为2至5个碱基（低于杂交极限）的5′-一磷酸酯化的寡核苷酸，非常适合从重组蛋白中去除核酸，从而符合FDA关于核酸污染的指南。Benzonase迅速水解核酸的能力使该酶成为降低粘度、减少加工时间并提高蛋白质产量的绝佳选择。例如，该酶与 BugBuster 和 PopCulture 蛋白质提取试剂兼容，因此可以与这些试剂一起添加，以消除大肠杆菌提取物中的粘度并去除核酸。

该酶包括两个 30kDa 的亚基。它在pH值6-10之间，温度0-42°C 起作用，并需要1-2mM Mg2+激活。该酶在离子和非离子型洗涤剂、还原剂、PMSF（1 mM）、EDTA（1 mM）和尿素（相对活性取决于特定条件）的存在下也具有活性。>150 mM单价阳离子，>100 mM磷酸盐，>100 mM硫酸铵或>100 mM盐酸胍能抑制其活性。

可提供超纯Benzonase 核酸酶（>99%，SDS-PAGE）和纯Benzonase核酸酶（>90%），标准浓度为25-29 U/µl，高浓度（HC）为250U/lµ。两种制剂均不含可检测的蛋白酶，并且比活度> 1 × 106蛋白。纯度>99%的产品经过内毒素测试，并含有<0.25 EU/1000 units. The product is suppliedas a 0.2 µm filtered solution in 50% glycerol.Store at -20°C.

Total endotoxin:<0.25 EU/1,000 units.Purity: >99%（SDS-PAGE）。

应用

用于去除蛋白样品中的核酸。

生化/生理作用

消化天然或热变性的DNA和RNA。

其他说明

一个单位定义为在 30 分钟内导致ΔA₂₆₀为1.0的酶量，这对应于37 µg DNA的完全消化。

法律信息

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

毒性：标准处理（A）

储存分类代码

10 - Combustible liquids

WGK

WGK 2

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商品

Benzonase® Nuclease Q&A

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

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BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria

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Benzonase® Nuclease Q&A

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

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