General description
The pSTBlue-1 AccepTor Vector Giga Kits and pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles Competent Cells for increased transformation efficiency. AccepTor Vector Kits are designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3′-dA nucleotide overhangs. The vector enables direct ligation by virtue of single 3′-dU overhangs that anneal with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo.
The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.
The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.
Features and Benefits
- No restriction enzymes or special primers
- Direct ligation of PCR product with vector
- Compatible with polymerases that leave single 3´-dA overhangs
- Blue/white screening with pSTBlue-1 or pETBlue-1 vectors
- Simple protocol takes as little as 40 minutes from PCR product to plating transformants
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
This product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates
Toxicity: Multiple Toxicity Values, refer to MSDS
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