71296
PhosphoSafe蛋白质提取试剂
Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.
别名:
Protein Extraction Reagent
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
immunoblotting: suitable
application(s)
sample preparation
shipped in
dry ice
storage temp.
−70°C
Quality Level
Disclaimer
毒性：有毒（F）
Features and Benefits
- 提取过程中保留的磷酸化状态
- 与激酶测定相容
General description
PhosphoSafe提取缓冲液可有效地从哺乳动物和昆虫细胞中提取胞质蛋白，同时保持其磷酸化状态。该试剂含有与CytoBuster蛋白质提取试剂（Trim 2001）相同的配方，但还包括四种磷酸酶抑制剂：氟化钠、钒酸钠、β-甘油磷酸酯和焦磷酸钠。PhosphoSafe提取试剂与激酶测定、蛋白质相互作用分析和其他应用兼容。该试剂以方便的25 mL瓶装提供（71296-3为1瓶，71296-4为5瓶）。
Other Notes
Trim，J.E. and Sawyer，D.L.2001.inNovations12，5.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Adrienne R Gibson et al.
Cancer research, 80(7), 1401-1413 (2020-02-12)
Pharmacologic ascorbate treatment (P-AscH-, high-dose, intravenous vitamin C) results in a transient short-term increase in the flux of hydrogen peroxide that is preferentially cytotoxic to cancer cells versus normal cells. This study examines whether an increase in hydrogen peroxide is
商品
蛋白磷酸化是一种可逆的翻译后加工，在细胞内信号传递中有非常重要的功能。采用Western Blotting技术检测磷酸化蛋白是探讨信号传导中上游调控、下游功能调整、信息交流及反馈机制等的基本手段。我们提供专用的优质试剂和研究方法，帮助你获得准确、灵敏的磷酸化检测数据。
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