CN
manufacturer/tradename
Novagen®
储存条件
OK to freeze
运输
wet ice
储存温度
2-8°C
一般描述
ProteoExtract®跨膜蛋白质提取试剂盒（TM-PEK）提供了无洗涤剂的新型化学方法，可从哺乳动物细胞和组织中温和有效地提取跨膜蛋白，例如GPCR。膜蛋白中富含的级分和大蛋白复合物与酶测定、非变性和基于SDS的凝胶电泳、蛋白质印迹和免疫沉淀直接兼容。富含的级分适用于胰蛋白酶凝胶内消化后基于质谱的分析。该试剂盒包含用于蛋白质依赖性优化的两种不同提取试剂、蛋白酶抑制剂混合物和用于处理二十个样品的适当缓冲液（每个样品2-5 x 106个细胞或25-50 mg组织）。
应用
ProteoExtract® Transmembrane Protein Extraction Kit has been used: for extraction of protein from the apex segment of the colon in western blot analysis of Na+/H+ exchanger (NHE3) for extracting plasma membrane proteins
特点和优势
- No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
- Reagents are sufficient to process a total of 40 samples.
警告
毒性：有毒（F）
其他说明
Nakamura，E.，et al. 2008.Cancer Sci.99， 1390.
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
WGK
WGK 2
法规信息
常规特殊物品
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Hepatic Tmem30a Deficiency Causes Intrahepatic Cholestasis by Impairing Expression and Localization of Bile Salt Transporters
Liu L, et al.
The American Journal of Pathology, 187(12), 2775-2787 (2017)
Runtao Ding et al.
Frontiers in behavioral neuroscience, 14, 60-60 (2020-05-05)
The monocarboxylate transporters (MCTs) MCT1, MCT2, and MCT4 are essential components of the astrocyte-neuron lactate shuttle (ANLS), which is a fundamental element of brain energetics. Decreased expression of MCTs can induce cognitive dysfunction of the brain. In the present study
Impairment of electroneutral Na+ transport and associated downregulation of NHE3 contributes to the development of diarrhea following in vivo challenge with Brachyspira spp.
Cole B. Enns, et al.
The American Journal of Physiology, 318(2), G288?G297-G288?G297 (2020)
Huimin Luo et al.
Oncology reports, 42(3), 1237-1247 (2019-07-20)
Although cisplatin is one of the most accepted therapies for ovarian cancer, recurrence and drug resistance remain problematic. Both the ubiquitin‑proteasome system (UPS) and connexin (Cx) are closely related to tumor progression. However, the role of ubiquitin‑specific protease 14 (USP14) and Cx
Asahi Takeuchi et al.
Nutrients, 12(9) (2020-09-24)
IIAEK (Ile-Ile-Ala-Glu-Lys, lactostatin) is a novel cholesterol-lowering pentapeptide derived from bovine milk β-lactoglobulin. However, the molecular mechanisms underlying the IIAEK-mediated suppression of intestinal cholesterol absorption are unknown. Therefore, we evaluated the effects of IIAEK on intestinal cholesterol metabolism in a
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