H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins a damaged site. H2AX is phosphorylated soon after the appearance of double-stranded breaks. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.

~ 23 kDa

Epitope: Lys119

Linear peptide containing ubiquitylated Lys119 of human Histone H2A.X.

Anti-Ubiquityl Histone H2A.X (Lys119) Antibody is a rabbit polyclonal antibody for detection of Ubiquityl Histone H2A.X (Lys119) also known as H2AXK119Ub, Histone H2A.X Ubiquityl K119) & has been validated in WB, IP, ICC.

Immunocytochemsitry Analysis: 1:500 dilution from a representative lot detected Histone H2A.X in RAW264.7 and NIH/3T3 cells.

Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This antibody recognizes Histone H2A.X when ubiquitylated at Lys119.

Unpurified

Unpurified rabbit polyclonal containing serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.

Control
HeLa acid extract

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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