AB10181
Anti-Pumilio-2 (PUM2) Antibody
from rabbit, purified by affinity chromatography
别名:
pumilio homolog 2 (Drosophila), pumilio homolog 2, pumilio (Drosphila) homolog 2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
immunohistochemistry
western blot
immunohistochemistry
western blot
Species reactivity:
rhesus macaque, mouse, human
Citations:
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-Pumilio-2 (PUM2) Antibody, from rabbit, purified by affinity chromatography
Quality Level
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rhesus macaque, mouse, human
species reactivity (predicted by homology)
chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), canine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PUM2(23369)
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected Pumilio-2 in human testis and teratoma tissue.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells.
Detect Pumilio-2 (PUM2) using this Anti-Pumilio-2 (PUM2) Antibody validated for use in IC, IH & WB.
Biochem/physiol Actions
This antibody recognizes Pumilio-2 near the N-terminus.
Analysis Note
Control
Mouse embryonic stem cell lysate
Mouse embryonic stem cell lysate
Evaluated by Western Blot on mouse embryonic stem cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
PUM2 is a member of the Puf family of RNA-binding proteins, which are believed to play a role in the regulation of posttranscriptional gene expression. Members of the Puf family of RNA binding proteins function as repressors of translation when bound to the 3-untranslated region of RNA transcripts.
~120 kDa observed.
Non specific band may be observed at 48 kDa
Non specific band may be observed at 48 kDa
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-2.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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