产品名称
抗血清素抗体, serum, Chemicon®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
shipped in
dry ice
target post-translational modification
unmodified
Application
抗-血清素抗体可检测血清素水平& 经发布 & 验证可用于IH。
研究子类别
神经递质& 受体
神经递质& 受体
研究类别
神经科学
神经科学
免疫组化:ABC或增强检测的1:1,000-1:2,500。需要戊二醛固定。抗体不会与未经戊二醛处理的组织发生反应。 应使用含有0.1-0.2%triton X-100的血清或BSA进行封闭;在一抗孵育过程中去除全部封闭和去污剂。仅在PBS或TBS中稀释以获得最佳结果。 对于荧光检测,必须减少抗体稀释,但由于戊二醛自发荧光,因此不建议使用。
最佳工作稀释度必须由最终用户进行确定。
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
血清素(5-羟色胺)
交叉反应性通过ELISA试验通过与以下化合物的竞争实验确定:
化合物 交叉反应性
5-羟色胺-G-PL 1
5-甲氧基色胺-G-PL 1/25
色胺-G-PL 1/60
5-羟色氨酸-G-PL 1/175
5-羟色胺 1/1150
色氨酸-G-PL 1/3400
5-甲氧基色氨酸-G-PL 1/19800
还使用自由浮动PAP技术在大鼠
中缝核上测试了抗血清的特异性。
缩写:
(G)戊二醛
(=)非还原偶联物
(PL)聚赖氨酸
交叉反应性通过ELISA试验通过与以下化合物的竞争实验确定:
化合物 交叉反应性
5-羟色胺-G-PL 1
5-甲氧基色胺-G-PL 1/25
色胺-G-PL 1/60
5-羟色氨酸-G-PL 1/175
5-羟色胺 1/1150
色氨酸-G-PL 1/3400
5-甲氧基色氨酸-G-PL 1/19800
还使用自由浮动PAP技术在大鼠
中缝核上测试了抗血清的特异性。
缩写:
(G)戊二醛
(=)非还原偶联物
(PL)聚赖氨酸
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
5-羟色胺-戊二醛-聚赖氨酸
Physical form
兔抗血清。 含0.05%叠氮化钠的液体。
Preparation Note
自收到之日起,在 -20°C 以未稀释的等分试样可保存 6 个月。应避免反复冻/融循环。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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