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Merck
CN

AB125

抗血清素抗体

serum, Chemicon®

别名:

5-HT

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

抗血清素抗体, serum, Chemicon®

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

shipped in

dry ice

target post-translational modification

unmodified

Application

抗-血清素抗体可检测血清素水平& 经发布 & 验证可用于IH。
研究子类别
神经递质& 受体
研究类别
神经科学
免疫组化:ABC或增强检测的1:1,000-1:2,500。需要戊二醛固定。抗体不会与未经戊二醛处理的组织发生反应。 应使用含有0.1-0.2%triton X-100的血清或BSA进行封闭;在一抗孵育过程中去除全部封闭和去污剂。仅在PBS或TBS中稀释以获得最佳结果。 对于荧光检测,必须减少抗体稀释,但由于戊二醛自发荧光,因此不建议使用。



最佳工作稀释度必须由最终用户进行确定。

Biochem/physiol Actions

血清素(5-羟色胺)

交叉反应性通过ELISA试验通过与以下化合物的竞争实验确定:



化合物 交叉反应性

5-羟色胺-G-PL 1

5-甲氧基色胺-G-PL 1/25

色胺-G-PL 1/60

5-羟色氨酸-G-PL 1/175

5-羟色胺 1/1150

色氨酸-G-PL 1/3400

5-甲氧基色氨酸-G-PL 1/19800



还使用自由浮动PAP技术在大鼠

中缝核上测试了抗血清的特异性。



缩写:



(G)戊二醛

(=)非还原偶联物

(PL)聚赖氨酸

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Immunogen

5-羟色胺-戊二醛-聚赖氨酸

Physical form

兔抗血清。 含0.05%叠氮化钠的液体。

Preparation Note

自收到之日起,在 -20°C 以未稀释的等分试样可保存 6 个月。应避免反复冻/融循环。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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