产品名称
Anti-Nerve Growth Factor-β Antibody, serum, Chemicon®
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, chicken, human
should not react with
bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NGF(4803)
Application
Immunohistochemistry at 1:500-1:5,000 (see suggested protocol).
Immunoblotting at 1:500-1:5,000.
ELISA at 1:500-1:5,000.
Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: This antibody will completely inhibit the survival and neurite outgrowth actions of mouse NGF in chicken dorsal root ganglion neurons in vitro.
Use neat for in vivo animal studies.
Optimal working dilutions must be determined by end user.
Immunoblotting at 1:500-1:5,000.
ELISA at 1:500-1:5,000.
Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: This antibody will completely inhibit the survival and neurite outgrowth actions of mouse NGF in chicken dorsal root ganglion neurons in vitro.
Use neat for in vivo animal studies.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
This Anti-Nerve Growth Factor-β Antibody is validated for use in ELISA, WB, IH, FUNC for the detection of Nerve Growth Factor-β.
Biochem/physiol Actions
Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human NT3, NT4 and BDNF by ELISA.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Mouse nerve growth factor-beta purified from salivary glands.
Other Notes
Replaces: 04-1119
Physical form
Whole rabbit antisera. Lyophilized. Reconstitute with 250 μL of sterile distilled water.
Preparation Note
Maintain at -20°C to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Tsia-Shu Lo et al.
The journal of obstetrics and gynaecology research, 43(1), 173-178 (2016-10-21)
By investigating the association of urodynamics and urogenital nerve growth factor (NGF) levels in vaginal mesh surgery, we may be able to associate the likelihood of postoperative lower urinary tract symptoms developing as a result of synthetic mesh implanted for
Sashwati Roy et al.
Nanomedicine : nanotechnology, biology, and medicine, 28, 102220-102220 (2020-05-19)
This work rests on our recent report on the successful use of tissue nanotransfection (TNT) delivery of Ascl1, Brn2, and Myt1l (TNTABM) to directly convert skin fibroblasts into electrophysiologically active induced neuronal cells (iN) in vivo. Here we report that
F Jardí et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 24(12), e580-e590 (2012-10-18)
Nerve growth factor (NGF)-mucosal mast cell (MMC) interaction has been implicated in the remodeling of enteric circuitries and associated functional changes. We investigated the involvement of NGF and its receptor TrkA in the altered colonic contractile activity observed in the
A Vega et al.
Developmental neurobiology, 76(8), 832-846 (2015-11-13)
Neurons are able to segregate transmitters to different axon endings. Segregation is a plastic neuronal feature; it can be modulated by synaptic environment. We have demonstrated that neurotrophin and other cellular factors regulate segregation in sympathetic neurons in culture. Herein
Daniel Aridgides et al.
PloS one, 8(2), e57450-e57450 (2013-02-26)
Cardiomyocytes express neurotrophin receptor TrkA that promotes survival following nerve growth factor (NGF) ligation. Whether TrkA also resides in cardiac fibroblasts (CFs) and underlies cardioprotection is unknown. To test whether CFs express TrkA that conveys paracrine signals to neighbor cardiomyocytes
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