biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
chicken, human, mouse, rat
should not react with
bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NGF(4803)
Immunogen
Mouse nerve growth factor-beta purified from salivary glands.
Application
Immunohistochemistry: 1-2 μg/mL (see suggested protocol).
Immunoblotting: 1-2 μg/mL
Bioassays (Inhibition of biological activity in vitro): 1-2 μg/mL
in vivo animal studies: use neat
ELISA: 1-2 μg/mL
Optimal working dilutions must be determined by the end user.
BIOLOGICAL ACTIVITY:
This antibody completely inhibits neuron survival and the outgrowth actions of mouse NGF in chicken dorsal root ganglion in vitro.
Immunoblotting: 1-2 μg/mL
Bioassays (Inhibition of biological activity in vitro): 1-2 μg/mL
in vivo animal studies: use neat
ELISA: 1-2 μg/mL
Optimal working dilutions must be determined by the end user.
BIOLOGICAL ACTIVITY:
This antibody completely inhibits neuron survival and the outgrowth actions of mouse NGF in chicken dorsal root ganglion in vitro.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
This Anti-Nerve Growth Factor-β Antibody, pain is validated for use in ELISA, WB, IH, FUNC for the detection of Nerve Growth Factor-β.
Biochem/physiol Actions
Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human BDNF, NT3 and NT4 by ELISA.
Physical form
Affinity purified immunoglobulin. Lyophilized from PBS. Contains no preservative. Reconstitute to desired volume with sterile distilled water. Suggested concentration 0.5-1.0 mg/mL.
Preparation Note
Maintain at lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.
Other Notes
Replaces: 04-1119
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brains.
Zhang, HT; Li, LY; Zou, XL; Song, XB; Hu, YL; Feng, ZT; Wang, TT
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
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