产品名称
Anti-Nerve Growth Factor-β Antibody, Chemicon®, from sheep
biological source
sheep
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
chicken, human, rat, mouse
should not react with
bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NGF(4803)
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
Anti-Nerve Growth Factor-β Antibody is an antibody against Nerve Growth Factor-β for use in ELISA, WB, IH.
Immunohistochemistry: 1-10 μg/mL (see suggested protocol).
Immunoblotting: 1-10 μg/mL
ELISA: 1-10 μg/mL
Inhibition of biological activity in vitro: 1-10 μg/mL
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.
Optimal working dilutions must be determined by the end user.
Immunoblotting: 1-10 μg/mL
ELISA: 1-10 μg/mL
Inhibition of biological activity in vitro: 1-10 μg/mL
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
Biochem/physiol Actions
Nerve growth factor-beta (NGF). By one site ELISA, less than 1% cross-reactivity against recombinant human BDNF or neurotrophin 4. Approximately 2% cross-reactivity against neurotrophin 3.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Mouse nerve growth factor-beta purified from salivary glands. Antigen purity greater than 95% by PAGE.
Other Notes
Replaces: 04-1119
Physical form
Format: Purified
Protein G purified immunoglobulin. Lyophilized from PBS, no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
Preparation Note
Maintain at -20 to -70°C in undiluted aliquots for up to one year. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
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存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
The role of mesopontine NGF in sleep and wakefulness.
Ramos, OV; Torterolo, P; Lim, V; Chase, MH; Sampogna, S; Yamuy, J
Brain Research null
Sravya Kurapati et al.
Journal of virology, 91(17) (2017-06-24)
Mechanisms of neuronal infection by varicella-zoster virus (VZV) have been challenging to study due to the relatively strict human tropism of the virus and the paucity of tractable experimental models. Cellular mitogen-activated protein kinases (MAPKs) have been shown to play
Martin Larhammar et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(46), 11074-11084 (2017-10-11)
The c-Jun-N-terminal kinase (JNK) signaling pathway regulates nervous system development, axon regeneration, and neuronal degeneration after acute injury or in chronic neurodegenerative disease. Dual leucine zipper kinase (DLK) is required for stress-induced JNK signaling in neurons, yet the factors that
Tomohiko Sadaoka et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(17), E2403-E2412 (2016-04-15)
Varicella-zoster virus (VZV) establishes latency in human sensory and cranial nerve ganglia during primary infection (varicella), and the virus can reactivate and cause zoster after primary infection. The mechanism of how the virus establishes and maintains latency and how it
Kelly Barford et al.
Scientific reports, 8(1), 4715-4715 (2018-03-20)
The development of the peripheral nervous system relies on long-distance signaling from target organs back to the soma. In sympathetic neurons, this long-distance signaling is mediated by target derived Nerve Growth Factor (NGF) interacting with its axonal receptor, TrkA. This
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