biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Aqp5(11830)
rat ... Aqp5(25241)
General description
水通道蛋白(AQP)是一个通道蛋白家族,可以让水或非常小的溶质通过,它们在需要快速、受控地运输液体的组织(如肾脏、肺和唾腺)中发挥作用。水通道蛋白5在唾液分泌中跨质膜的水移动和角膜/晶状体润滑中起重要作用,也是阐明糖尿病性口腔干燥机制的研究的焦点。
观测值〜28 kDa
Immunogen
对应于大鼠水通道蛋白5的胞质结构域的KLH偶联的线性肽。
表位:细胞质结构域
Application
免疫组化分析:一个代表性批次的1:500-2,000稀释液在大鼠肺和小鼠气管组织中检测到水通道蛋白5。
免疫组织化学分析:一个来自独立实验室的代表性批次在多聚甲醛固定的各种组织中检测到水通道蛋白5(参见参考文献)。
免疫组织化学分析:一个来自独立实验室的代表性批次在多聚甲醛固定的各种组织中检测到水通道蛋白5(参见参考文献)。
研究子类别
发育信号转导
发育信号转导
研究类别
神经科学
神经科学
该抗水通道蛋白5(AQP5)抗体经验证可用于WB以检测水通道蛋白5。
Biochem/physiol Actions
水通道蛋白5(AQP5)通过蛋白质印迹,该抗体与大鼠颌下腺裂解液中约 24 kDa 的条带反应。
Physical form
免疫亲和纯化
纯化兔多克隆溶于含有0.05%叠氮化钠PBS的缓冲液中。
Preparation Note
自收到之日起,在2-8°C以未稀释的等分试样可保存1年。
在运输过程中,少量产品有时会夹在产品瓶的密封中。对于体积为200μL或更小的产品,我们建议在小瓶的硬质表面上轻轻敲打,或在台式离心机中短暂离心以收集容器′盖中的所有液体。
在运输过程中,少量产品有时会夹在产品瓶的密封中。对于体积为200μL或更小的产品,我们建议在小瓶的硬质表面上轻轻敲打,或在台式离心机中短暂离心以收集容器′盖中的所有液体。
Analysis Note
对照
对大鼠唾液腺裂解液进行检测
对大鼠唾液腺裂解液进行检测
通过蛋白质印迹对大鼠唾液腺组织裂解液进行了评估。
蛋白质印迹分析:0.1 µg/mL的该抗体可在10 µg大鼠唾液腺组织裂解中检测到水通道蛋白5。
蛋白质印迹分析:0.1 µg/mL的该抗体可在10 µg大鼠唾液腺组织裂解中检测到水通道蛋白5。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Yao Chen et al.
Respiratory research, 13, 1-1 (2012-01-05)
Distal alveolar morphogenesis is marked by differentiation of alveolar type (AT)-II to AT-I cells that give rise to the primary site of gas exchange, the alveolar/vascular interface. Endothelial-Monocyte Activating Polypeptide (EMAP) II, an endogenous protein with anti-angiogenic properties, profoundly disrupts
Verification and spatial localization of aquaporin-5 in the ocular lens.
Grey, AC; Walker, KL; Petrova, RS; Han, J; Wilmarth, PA; David, LL; Donaldson, PJ; Schey, KL
Experimental Eye Research null
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