产品名称
抗囊泡单胺转运蛋白2抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SLC18A2(6571)
Preparation Note
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始小瓶进行离心。
Analysis Note
对照
大鼠脑组织
大鼠脑组织
Application
抗囊泡单胺转运蛋白2抗体是一种抗囊泡单胺转运蛋白2的抗体,用于ELISA、IH & WB。
研究子类别
离子通道 & 转运体
离子通道 & 转运体
研究类别
神经科学
神经科学
蛋白质印迹:1-10 μg/mL(化学发光技术)
免疫组织化学:1-20 μg/mL,在多聚甲醛固定切片上。
ELISA:0.5-1.0 μg/mL(1 μg/mL VMAT1肽(目录编号AG251)/孔)
最佳工作稀释度必须由最终用户进行确定。
免疫组织化学:1-20 μg/mL,在多聚甲醛固定切片上。
ELISA:0.5-1.0 μg/mL(1 μg/mL VMAT1肽(目录编号AG251)/孔)
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
可识别囊泡单胺转运蛋白2(VMAT2)。针对C-末端VMAT2肽制备的抗体在用VMAT2转染的CHO的核后上清液中检测到约55 kDa的主要条带,而在野生型细胞中未检测到(Peter et al.1995;Nirenberg et al.1995)。还检测到一些其他条带,包括较高和较低的分子量,并且不受蛋白酶抑制剂的影响。较高的分子量带可代表糖基化的VMAT2(Peter et al. 1995; Nirenberg et al. 1995, Liu et al. 1994)。VMAT2已定位于肾上腺嗜铬细胞、交感神经腱鞘囊肿细胞、胃的肠嗜铬样细胞和CNS中(Peter et al. 1995; Nirenberg et al. 1995; Nirenberg et al. 1996)。 由于大鼠和人VMAT2之间92%的同源性,可能识别人。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
55 kDa
Immunogen
来自大鼠VMAT2的13个氨基酸的C-末端肽序列(Liu et al.1992)。预计该肽是细胞质的。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Physical form
免疫亲和纯化
含0.1% BSA的PBS溶液。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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