biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
should not react with
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... FAS(355)
Immunogen
An 18 amino acid peptide sequence (aa 130-146) from rat FASL. This sequence is known to be 94% homologous with mouse FASL, differing by one amino acid. This peptide region does not show significant homology with human FASL, with only four amin
Application
Detect Fas Ligand using this Anti-Fas Ligand Antibody validated for use in ELISA & WB.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western blotting:1:1000 - 1:5,000, using Chemiluminescent detection. The use of 0.1 - 0.5% milk in diluents is recommended to reduce background. Based upon the calculated amino acid sequences, rat (278), mouse (279), and human (281) FASL has a molecular weight of ~32 kDa. A 40 kDa FASL band has been detected in mouse T cells.
ELISA: 1:10,000 - 1:50,000, for detection of control peptide (AG626) coated at a 1 μg/mL concentration.
Optimal working dilutions must be determined by end user.
ELISA: 1:10,000 - 1:50,000, for detection of control peptide (AG626) coated at a 1 μg/mL concentration.
Optimal working dilutions must be determined by end user.
Physical form
Liquid antiserum containing 0.05% sodium azide as a preservative.
Preparation Note
Store at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze-thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 1