Anti-Ubiquitin Antibody

Purified uboquitin covalently coupled to KLH using glutaraldehde.

Anti-Ubiquitin Antibody detects level of Ubiquitin & has been published & validated for use in WB, IH, IH(P).

Immunohistochemistry: 1:1,000-1:5,000. Tissue fixed in 10% formalin, Bouin′s fixative for 1-2 days and embedded in paraffin, or unfixed frozen tissues may be used.

Immunoblotting: 1:1,000-1:5,000.

Optimal working dilutions must be determined by end user.

Research Category
Protein Trafficking

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Ubiquitin (76 amino acid, 8.5 kDa). Shows staining of neurofibrillary tangles, neurites in senile plaques and a subset of neuronal processes. No reactivity seen to tau, neurofilament or microtubule-associated proteins.

Neat rabbit antisera with 10 mM sodium azide.

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

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