生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, mouse
制造商/商品名称
Chemicon®
技术
western blot: suitable
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... APAF1(317)
特异性
Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and was designated Apaf1 for apoptosis protease-activating factor 1(Zou et al. 1997; Cecconi et al. 1998). Apaf1 binds to cytochrome C (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 which is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Li et al. 1997). Apaf1 can also associate with caspase-4 and caspase-8 (Hu et al. 1998). Apaf1 is ubiquitously expressed in human tissues (Zou et al. 1997).
免疫原
Epitope: N-terminus
Polyclonal antibody raised against a peptide corresponding to amino acids 12 to 28 of human Apaf1 (Zou et al. 1997) The sequences of the immunogenic peptide are identical between human and mouse (Zou et al. 1997; Cecconi et al. 1998).
应用
Anti-Apaf-1 Antibody, N-terminus is an antibody against Apaf-1 for use in WB.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western blot: 1:1000 to 1:4000
Whole cell lysate from HeLa cells can be used as a positive control and a 130 kDa band should be detected. Optimal working dilutions must be determined by end user.
Whole cell lysate from HeLa cells can be used as a positive control and a 130 kDa band should be detected. Optimal working dilutions must be determined by end user.
联系
Replaces: 04-579
外形
Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Format: Purified
储存及稳定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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