Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain

Specific for Dopamine Receptor D3. Human Dopamine D3 receptor is a 400 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant sequence similarity with other dopamine receptors (D1, D2, D4 or D5).

A 19 amino acid peptide sequence from the human D3 receptor within the 3rd cytoplasmic domain.

Epitope: cytoplasmic domain

Detect Dopamine D3 Receptor using this Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain validated for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blot: 1-10 μg/mL (Chemiluminescence technique) Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band.

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Cat. Number AG224)/well)

Optimal working dilutions must be determined by the end user.

Affinity purified immunoglobulin. Liquid in PBS, pH 7.5 with 0.1% BSA. No preservatives

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

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