Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain

Epitope: cytoplasmic domain

A 19 amino acid peptide sequence from the human D3 receptor within the 3rd cytoplasmic domain.

Detect Dopamine D3 Receptor using this Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain validated for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blot: 1-10 μg/mL (Chemiluminescence technique) Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band.

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Cat. Number AG224)/well)

Optimal working dilutions must be determined by the end user.

Specific for Dopamine Receptor D3. Human Dopamine D3 receptor is a 400 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant sequence similarity with other dopamine receptors (D1, D2, D4 or D5).

Affinity purified immunoglobulin. Liquid in PBS, pH 7.5 with 0.1% BSA. No preservatives

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

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