Anti-MMP-2 Antibody, whole molecule

72 kDa

MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer of the stromal cells. There is a co-operation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.

A synthetic peptide from the second half of human MMP-2.

Epitope: Whole molecule

Detect MMP-2 using this Anti-MMP-2 Antibody, whole molecule validated for use in IH(P) & WB.

Immunohistochemistry (formalin/paraffin):
10-20 µg/mL of a previous lot was kept for 30 min at RT. No special pretreatment is required for staining formalin-fixed paraffin-embedded sections.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

AB19167 recognizes protein at 72 kDa, identified as matrix metalloproteinase 2. MMP-2 is also known as 72 kDa collagenase IV or gelatinase A. It is synthesized as a 631 amino acid proenzyme that is activated by cleavage of the first 80 amino acids. AB19167 shows no cross-reaction with (pro) and active forms of other MMPs. Cytoplasmic localization.

Reactivity with other species has not been confirmed.

Protein A chromatography

Format: Purified

Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Stable for 1 year at 2-8ºC from date of receipt.

Control
Positive control: Conditioned, serum-free medium from TPA-treated human fetal lung (HFL-1) cells. Placenta or bladder, breast, or ovarian carcinomas.

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected MMP-2 on 10 µg of PC12 lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1048

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