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Merck
CN

AB2953

Anti-ATG3 Antibody

from rabbit, purified by affinity chromatography

别名:

ATG3 autophagy related 3 homolog (S. cerevisiae), APG3 autophagy 3-like (S. cerevisiae), autophagy-related protein 3, autophagy Apg3p/Aut1p-like, APG3 autophagy 3-like, Protein PC3-96

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
-
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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... ATG3(64422)

General description

ATG3 is thought to be an autophagy-related ubiquitin carrier protein (E2)-like enzyme that is the go between for the conjugation on ATG8 and phosphatidylethanolamine (PE). This compound is necessary for autophagy, which is a process in which a cell is degraded by its own lysosomal process.
~37 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human ATG3.

Application

Anti-ATG3 Antibody detects level of ATG3 & has been published & validated for use in WB.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

Other homologies: Ape, rhesus macaque, equine, porcine, and bovine (99% sequence homology). Mouse (98% sequence homology). Rat (97% sequence homology).

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HL-60 cell lysate
Evaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ATG3 in 10 µg of HL-60 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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