Anti-Metadherin Antibody

Metadherin is a type 2 transmembrane protein who′s overexpression was first described in breast cancers. It plays an important role in the metastasis of breast cancers into the lungs as a secondary site of development. Antibodies reactive to the lung-homing domain of metadherin and siRNA-mediated knockdown of metadherin expression in breast cancer cells inhibited experimental lung metastasis, indicating that tumor cell metadherin mediates localization at the metastatic site (Brown, 2009).

~ 75 kDa

Epitope: Homing domain

Recombinant GST-tagged proteins corresponding at or around the lung homing domain of mouse Metadherin.

Anti-Metadherin Antibody is an antibody against Metadherin for use in WB & IC.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western blot Analysis: 0.25 µg/ml of this antibody detected Metadherin in 10 µg of HEK293, HepG2, and Jurkat cell lysates.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Metadherin in NIH/3T3 and HeLa cells.

The antibody recognizes Metadherin at and around the homing domain.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western blot in HeLa cell lysate.

Western blot Analysis: 0.25 µg/ml of this antibody detected Metadherin in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.