AB3071
抗水通道蛋白0抗体
Chemicon®, from rabbit
别名:
AQP0, MIP, Major Intrinsic Protein
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA
western blot
western blot
Species reactivity:
human
Citations:
18
Technique(s):
ELISA: suitable
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗水通道蛋白0抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MIP(4284)
Application
使用经验证可用于ELISA&WB的抗水通道蛋白0抗体检测水通道蛋白0。
我们建议在全部一抗/二抗稀释液中使用0.5-1%的乳汁，以抑制非特异性条带。
蛋白质印迹：使用化学发光技术为1-10μg/mL
ELISA：0.5-1.0 μg/mL
最佳工作稀释度必须由最终用户确定。
蛋白质印迹：使用化学发光技术为1-10μg/mL
ELISA：0.5-1.0 μg/mL
最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
水是全部活细胞的关键组成部分。有趣的是，组织膜显示出很大程度的透水性。哺乳动物的红细胞，肾近端小管和髓袢降支细段对水具有极高的渗透性。通过简单的扩散或通过特殊蛋白“水通道蛋白”介导的促进性运输机理，水穿过疏水性质膜。在过去的十年中，已经克隆，表达了水通道蛋白家族几个成员的基因，并在许多组织中研究了它们的分布。水通道蛋白0（或称MIP26，主要内源性蛋白26 kDa）以及水通道蛋白1（从红细胞纯化；也称CHIP-28（通道形成整合蛋白28 kD；268 AA； 基因位点7p14））已经成为了水通道蛋白生长家族的基础。晶状体特异性水通道蛋白0代表着总晶状体膜蛋白的80%。MIP26缺陷是常染色体显性白内障的原因。白内障Fraser突变（CAT-FR或枯萎型）是转位子诱导的剪接错误，取代了MIP C末端的长末端重复序列。晶状体浑浊突变（LOP）是一种AA取代，抑制了MIP对细胞膜的靶向。人水通道蛋白0是一种263个氨基酸的跨膜蛋白，属于MIP家族。水通道蛋白家族预计含有六个跨膜结构域。预计N末端和C末端位于细胞质。
Immunogen
人水通道蛋白0 的羧基末端结构域中的17个氨基酸的合成肽（Shiels et al. 1988; Kent et al. 1990; Pisano et al. 1991; Shiels et al. 1996）被选用于抗体生产。预计该该结构域位于细胞质。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Yuefang Zhou et al.
Differentiation; research in biological diversity, 102, 1-9 (2018-05-26)
Ephrin type-A receptor 2 (EPHA2) and one of its ligands, ephrin-A5 (EFNA5), have been associated with loss of eye lens transparency, or cataract, - an important cause of visual impairment. Here we show that mice functionally lacking EPHA2 (Epha2-null), EFNA5
Yuefang Zhou et al.
Biochimica et biophysica acta, 1862(8), 1433-1442 (2016-05-08)
Major intrinsic protein (MIP) is a functional water-channel (AQP0) that also plays a key role in establishing lens fiber cell architecture. Genetic variants of MIP have been associated with inherited and age-related forms of cataract; however, the underlying pathogenic mechanisms
Thomas M Bennett et al.
Biochemical and biophysical research communications, 478(2), 988-993 (2016-08-16)
Major intrinsic protein or aquaporin-0 (MIP/AQP0) functions as a water channel and a cell-junction molecule in the vertebrate eye lens. Loss of MIP function in the lens leads to degraded optical quality and cataract formation by pathogenic mechanisms that are
Yichen Wang et al.
Investigative ophthalmology & visual science, 58(10), 3896-3922 (2017-08-02)
Previous research showed that the absence of β1-integrin from the mouse lens after embryonic day (E) 13.5 (β1MLR10) leads to the perinatal apoptosis of lens epithelial cells (LECs) resulting in severe microphthalmia. This study focuses on elucidating the molecular connections
Thuzar Thein et al.
Development (Cambridge, England), 143(21), 3994-4002 (2016-11-03)
Fibroblast growth factor (FGF) signaling is an essential regulator of lens epithelial cell proliferation and survival, as well as lens fiber cell differentiation. However, the identities of these FGF factors, their source tissue and the genes that regulate their synthesis
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