Recognizes NHE3.

The immunogen sequence shares 90% homology with mouse NHE3.

A 22 AA peptide within the cytoplasmic, c-terminal domain of the rat NHE3 (Brant et al. 1995; Orlowsji et al. 1992; Chris Yun et al. 1995; Josette & Pouyssegur 1995). Control peptide is available, cat# AG779.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-Na+/H+ Exchanger-3 Antibody is validated for use in ELISA, WB for the detection of Na+/H+ Exchanger-3.

Western blot: 1-10 μg/mL using Chemiluminescence technique. A band of approximately 90 kD has been detected in brush border membrane (Yohsioka et al. 1997). ELISA: 1:100,000 using 50-100 ng control peptide/well.

Optimal working dilutions must be determined by end user.

93 kDa

ImmunoAffinity Purified

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Kidney, jejunum, colon, and stomach tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.