Anti-Angiopoietin-2 Antibody, NT

20 amino acid peptide sequence near the amino-terminus of mouse Ang-2.

Epitope: N-terminus

Detect Angiopoietin-2 using this Anti-Angiopoietin-2 Antibody, N-terminus validated for use in ELISA & WB.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western blot: 1-10 μg/mL using ECL. The predicted size of Ang-2 is ~55 kDa. Like Ang-1, its size may higher due to glycosylation.

ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.

Recognizes mouse Angiopoietin-2 (Ang-2). Mouse/human Ang-2 is a 496 AA secreted angiogenic protein (Suri et al. 1996). There is ~85% homology between mouse and human Ang-2. It is only 60% homologous with Ang-1 (Davis et al. 1996). Ang-2 may act as an antagonist of Ang-1 and Tie-2 (Suri et al. 1996). It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

The immunogenic peptide sequence is 71% (15/21 aa) homologous with human Ang-2 and has no substantial homology with Ang-1. Antibody crossreactivity in other species is not established. The immunogen peptide is 85% conserved in porcine and 55% in chicken.

Affinity purified immunoglobulin presented as liquid in PBS containing 0.1% BSA and 0.05% azide, pH 7.5

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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