AB3202
抗-LIM-3 抗体
Chemicon®, from rabbit
别名:
Anti-HMFN1661
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC (p), IP, WB
Citations:
7
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
fish, frog, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ABLIM3(22885)
Immunogen
小鼠LIM-3蛋白的C末端部分。
Application
免疫组化：在石蜡包埋的切片上为1：250-1：1,000 。在冰冻切片上为1：3,000-1：6,000 。在全标本上为1：4,000-1：8,000。推荐固定剂MEMFA.
免疫细胞化学：1：200-1：500
蛋白质印迹：1：2,500-1：5,000
免疫沉淀：1：200
该抗体在免疫荧光中工作正常。使用偶联生物素的二抗，然后以链霉亲和素偶联至荧光染料。
最佳工作稀释度必须由最终用户进行确定。
免疫细胞化学：1：200-1：500
蛋白质印迹：1：2,500-1：5,000
免疫沉淀：1：200
该抗体在免疫荧光中工作正常。使用偶联生物素的二抗，然后以链霉亲和素偶联至荧光染料。
最佳工作稀释度必须由最终用户进行确定。
抗LIM-3抗体是针对LIM-3的抗体，可用于IP、WB、IC、IH、IH(P)。
研究子类别
发育神经科学
神经&胶质标志物
发育神经科学
神经&胶质标志物
研究类别
神经科学
神经科学
Biochem/physiol Actions
可识别LIM-3。该抗体不与组织切片中的gsh-4交叉反应，但是在蛋白质印迹和免疫沉淀中可能有交叉反应。
Physical form
形式：纯化
纯化的免疫球蛋白。 0.02M磷酸盐缓冲液，0.25M NaCl和0.1%叠氮化钠的液体。
Preparation Note
以未稀释的等分试样保存于2-8°C下6个月。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Mai Nakamura et al.
Cytotechnology, 68(3), 409-417 (2014-10-31)
Mouse embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have the ability to differentiate in vitro into various cell lineages including neurons. The differentiation of these cells into neurons has potential applications in regenerative medicine. Previously, we reported
Clifford R Hume et al.
Gene expression patterns : GEP, 7(7), 798-807 (2007-07-03)
A cascade of transcription factors is believed to regulate the coordinate differentiation of primordial inner ear cells into the subtypes of hair cells and supporting cells. While candidate genes involved in this process have been identified, the temporal and spatial
Stephanie W Fowler et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(23), 7871-7885 (2014-06-06)
An unresolved debate in Alzheimer's disease (AD) is whether amyloid plaques are pathogenic, causing overt physical disruption of neural circuits, or protective, sequestering soluble forms of amyloid-β (Aβ) that initiate synaptic damage and cognitive decline. Few animal models of AD
Anne-Laure Schang et al.
Molecular endocrinology (Baltimore, Md.), 27(1), 74-91 (2012-12-06)
GATA2 transcription factor and LIM homeodomain proteins Islet1 (ISL1) and LIM homeobox 3 (LHX3) are suspected to be involved in gonadotrope cell fate and maintenance. The GnRH receptor gene (Gnrhr), crucial for gonadotrope function, is expressed in the pituitary gland
Swetha K Godavarthi et al.
Proceedings of the National Academy of Sciences of the United States of America, 121(15), e2318041121-e2318041121 (2024-04-03)
Stable matching of neurotransmitters with their receptors is fundamental to synapse function and reliable communication in neural circuits. Presynaptic neurotransmitters regulate the stabilization of postsynaptic transmitter receptors. Whether postsynaptic receptors regulate stabilization of presynaptic transmitters has received less attention. Here
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