Anti-Aquaporin 3 Antibody

Highly purified peptide corresponding to residues 275-292 of rat Aquaporin 3 (Accession Number P47862) coupled to KLH.

Anti-Aquaporin 3 Antibody detects level of Aquaporin 3 & has been published & validated for use in WB, IH.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blotting: 1:200 on rat kidney membranes. Identifies a 31kDa and 40kDa band. The large band may represent the glycosylated form of the protein.

Immunohistochemistry on mouse kidney sections.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Recognizes Aquaporin 3.

SPECIES REACTIVITIES: The epitope specific for Aquaporin 3 is highly homologous in human and sheep (15/18). Reactivity with other species has not yet been tested.

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.