biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AQP3(360)
Immunogen
Highly purified peptide corresponding to residues 275-292 of rat Aquaporin 3 (Accession Number P47862) coupled to KLH.
Application
Anti-Aquaporin 3 Antibody detects level of Aquaporin 3 & has been published & validated for use in WB, IH.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blotting: 1:200 on rat kidney membranes. Identifies a 31kDa and 40kDa band. The large band may represent the glycosylated form of the protein.
Immunohistochemistry on mouse kidney sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on mouse kidney sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Aquaporin 3.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 3 is highly homologous in human and sheep (15/18). Reactivity with other species has not yet been tested.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 3 is highly homologous in human and sheep (15/18). Reactivity with other species has not yet been tested.
Physical form
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Preparation Note
Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品?
试试我们的产品选型工具.
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Takashi Nagai et al.
Neurourology and urodynamics, 38(6), 1560-1570 (2019-06-14)
To measure the effects of nicotine on lower urinary tract symptoms (LUTS), bladder blood flow, and the urothelial markers hypoxia-inducible factor 1α (HIF1α), uroplakin III (UPIII), and aquaporin 3 (AQP3). Ten-week-old female Sprague Dawley rats were subcutaneously injected with 2 mg/kg
Harumi Hisaki et al.
American journal of physiology. Renal physiology, 303(7), F1049-F1059 (2012-07-27)
Saposin D-deficient (Sap-D(-/-)) mice develop polydipsia/polyuria and die prematurely due to renal failure with robust hydronephrosis. Such symptoms emerged when they were around 3 mo of age. To investigate the pathogenesis of their water mishandling, we attempted to limit water
Aquaporin3 is a sperm water channel essential for postcopulatory sperm osmoadaptation and migration.
Qi Chen et al.
Cell research, 21(6), 922-933 (2010-12-08)
In the journey from the male to female reproductive tract, mammalian sperm experience a natural osmotic decrease (e.g., in mouse, from ~415 mOsm in the cauda epididymis to ~310 mOsm in the uterine cavity). Sperm have evolved to utilize this
Jae Mi Suh et al.
Molecular endocrinology (Baltimore, Md.), 20(12), 3412-3420 (2006-09-16)
Nuclear receptors are transcriptional regulators that play important roles in embryonic development and organogenesis. To study the potential roles of nuclear receptors in kidney development, we examined the expression patterns of a subset of nuclear receptors in which specific antibodies
Kyungmi Yang et al.
Marine drugs, 18(8) (2020-07-28)
Radiation dermatitis (RD) is one of the most common side effects of radiotherapy; its symptoms progress from erythema to dry and moist desquamation, leading to the deterioration of the patients' quality of life. Active metabolites in brown seaweed, including phlorotannins
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持